Welcome to the Nature Products Discovery Tool Platform

AttB-find

AttB-find is a web tool to identify the attB integration sites in microbial genomes. It uses BLAST analysis to find potential attB sites based on sequence similarity and scoring thresholds.

RiPPs Analysis

Coming soon... Tools for analyzing Ribosomally synthesized and Post-translationally modified Peptides (RiPPs) in genomic data.

Other Tools

Access additional bioinformatics tools and utilities for comprehensive genomic analysis and data processing.
Coming soon...

Issues and Questions

Please submit any issues or questions that you may have.
Email: zhengjie.liu@sjtu.edu.cn

Cite our work

Please cite our work if you use this platform in your research:

Development of a versatile chassis for the efficient production of diverse type II polyketides.
Zou Y, Wang R, Liu Z, Zou Y, Zeng S, Fang M, Chen H, Wang P, Xie C, Deng Z, Zhang F, Liu R, Liu T. Nat Commun. 2025 Aug 8;16(1):7321. doi: 10.1038/s41467-025-62659-0.

References

Sequences in attB that affect the ability of phiC31 integrase to synapse and to activate DNA cleavage.
Gupta M, Till R, Smith MC. Nucleic Acids Res. 2007;35:3407-19. doi: 10.1093/nar/gkm206.

New ΦBT1 site-specific integrative vectors with neutral phenotype in Streptomyces.
Gonzalez-Quiñonez N, López-García MT, Yagüe P, Rioseras B, Pisciotta A, Alduina R, Manteca Á. Appl Microbiol Biotechnol. 2016;100:2797-808. doi: 10.1007/s00253-015-7271-0.

aMSGE: advanced multiplex site-specific genome engineering with orthogonal modular recombinases in actinomycetes.
Li L, Wei K, Liu X, Wu Y, Zheng G, Chen S, Jiang W, Lu Y. Metab Eng. 2019;52:153-167. doi: 10.1016/j.ymben.2018.12.001.

Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.
Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD. Nat Biotechnol. 2023;41:488-499. doi: 10.1038/s41587-022-01494-w.

AttB-find Analysis

AttB Site Identification

Find attB integration sites in microbial genomes using BLAST analysis. Please upload a FASTA file containing your genome sequence:

Click to upload or drag and drop FASTA files (.fasta, .fa, .fas, .fna) up to 100MB

RiPPs Analysis

Peptide Identification

Identify and analyze ribosomally synthesized and post-translationally modified peptides in your genomic data.

Other Tools

Available Utilities

Access a variety of bioinformatics tools and utilities for your research needs.

Data Visualization

Create charts and graphs from your analysis results

Data Export

Export results in various formats

Settings

Configure analysis parameters